Standard operating procedure for determining the ability of PBO to restore susceptibility of adult mosquitoes to pyrethroid insecticides in WHO tube tests

The purpose of this standard operating procedure (SOP) is to describe the process for evaluating the ability of PBO to restore the susceptibility of mosquitoes to pyrethroids in pyrethroid-resistant vector populations. This bioassay provides an indirect assessment of the involvement of mono-oxygenases in conferring pyrethroid resistance, and results are meant to contribute towards decision-making on the deployment of pyrethroid-PBO nets.