COLLECTION

Resources for Malaria Molecular Surveillance

LABORATORY PROCEDURES

Sample preparation; Laboratory protocols and SOPs

DNA extraction

Dried Blood Spot Extraction (MalariaGEN)

Standard Operating Procedure for Dried Blood Spot (DBS) Extraction: Vacuum methodology

Extraction of DNA from DBS and RDTs in 96 well plates (ISGlobal)

Extraction of DNA from dried blood spots S (DBS) and rapid diagnostic tests (RDT) by Chelex method in 96 well plates

Amplicon Sequencing

Amplicon toolkit (MalariaGEN)

The MalariaGEN Amplicon Sequencing Toolkit provides partners with the checklists, protocols, and analytical methods needed to sequence and investigate targeted genes associated with drug or insecticide resistance

CDC - Malaria Resistance Surveillance (MaRS) Protocol

Standard Operating Procedure (SOP) describing how to prepare and sequence the full length P. falciparum genes associated with antimalarial resistance on the Illumina MiSeq

MAD4HatTeR Amplicon Sequencing Protocol (EPPIcenter)

The Multiplex Amplicons for Drug, Diagnostic, Diversity, and Differentiation Haplotypes via Targeted Resequencing (MAD4HatTeR) platform is an Illumina-compatible, modular sequencing panel for P. falciparum with the ability to genotype low density, polyclonal field samples.

NMEC Oxford Malaria Amplicon Drug-resistance Sequencing (NOMADS) Protocol

Cost-effective targeted nanopore sequencing of P. falciparum malaria

PF AmpliSeq Custom Assay Protocol (ITM Antwerp)

Multiplex PCR approach to amplify all P. falciparum genomic regions of interest in a rapid and easily standardizable procedure and allows simultaneous amplification of a high number of targets at once.

Rapid Multiplexed Nanopore Amplicon Sequencing of P. falciparum Microhaplotype Loci Protocol (Swiss TPH)

Multiplexed nanopore AmpSeq panel targeting six highly diverse P. falciparum microhaplotype loci, capable to provide rapid PCR-corrected drug failure estimates.
More details are available in this pre-print article.