PF AmpliSeq Custom Assay Protocol (ITM Antwerp)

Multiplex PCR approach to amplify all P. falciparum genomic regions of interest in a rapid and easily standardizable procedure and allows simultaneous amplification of a high number of targets at once.

• SOP: AmpliSeq custom assay for molecular surveillance of Plasmodium falciparum and Plasmodium vivax parasites from dried blood spot DNA
  (Suggested citation: J.H. Kattenberg, ITM Malariology Laboratory procedures, 2023. AmpliSeq custom assay for molecular surveillance of Plasmodium falciparum and Plasmodium vivax parasites from dried blood spot DNA)

• Visual aids for ampliSeq Pf/Pv SOP

Related publication:
– Molecular Surveillance of Malaria Using the PF AmpliSeq Custom Assay for Plasmodium falciparum Parasites from Dried Blood Spot DNA Isolates from Peru

Related project (MESA Track):
– Development of tools and strategies for malaria molecular surveillance. Countries included: Peru, Vietnam, Rwanda, Burkina Faso and DRC