Last Updated: 31/01/2025
Genotyping methods to distinguish Plasmodium falciparum recrudescence from new infection for the assessment of antimalarial drug efficacy: an observational, single-centre, comparison study
Objectives
Distinguishing Plasmodium falciparum recrudescence from new infections is crucial for the assessment of antimalarial drug efficacy against P falciparum. We aimed to compare the efficacy of different genotyping methods to assess their effect on drug efficacy estimates, particularly in patients from high-transmission settings with polyclonal infections.
Swiss Tropical and Public Health Institute (Swiss TPH), Switzerland
To our knowledge, this study is the first to compare the most used genotyping methods to distinguish recrudescence from new infection including high-resolution capillary electrophoresis with either msp1, msp2, and glurp or microsatellites with the newly developed targeted amplicon deep sequencing (TADS) assay. In this study, we compared five genotyping methods to distinguish P falciparum recrudescence from new infection using the same P falciparum laboratory strain mixtures and patient samples performed by the same operator in a single laboratory.
May 2020 — Aug 2021
$15,547
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