Last Updated: 22/11/2024
Comprehensive analysis of parasite- and host-side multigene families related to malaria onset using blood from malaria patients in West Africa
Objectives
*Original title and text were machine translated from Japanese.
This study aims to elucidate the molecular mechanism underlying the onset of malaria in adults who already have acquired immunity, and analyze the gene expression of both the parasite and the host in the blood of malaria patients in Ghana.
Noguchi Memorial Institute for Medical Research (NMIMR), Ghana
In this joint research, the expression of multiple genes of the parasite will be analyzed, and the complete genome will be determined by long-read sequencer analysis using parasite mRNA and genomic DNA derived from the blood of malaria patients. Sample collection activities in Ghana will also be managed. This year, long-read RNA-seq will be optimized at domestic institutions before samples arrive from Ghana. Specifically, the minimum protozoan infection rate, blood volume, nucleic acid extraction method, and RNA/DNA purity and quantity necessary for analysis will be examined using an experimental system with laboratory strains. In addition, after selection, detailed discussions were promptly held with the Ghanaian-based faculty members and researchers from the Noguchi lab. Sample collection locations were selected, a sample collection protocol was created, and an ethics application was submitted to obtain permission for research activities locally. Sample collection will begin as soon as approval is granted.
Apr 2023 — Mar 2026
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