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Last Updated: 06/11/2024
An instrument-free malaria diagnostic
Objectives
This proposal sets to develop a sensitive, rapid, and affordable CRISPR-based diagnostic test for malaria parasites.
The assay will be simple to run, require no instrumentation or advanced training, and be suitable for resource-limited regions. In the Phase I, the multi- chambered FAST-CRISPR diagnostic will be designed and optimized using cultured parasites and plasmid DNA targets. Later in the Phase I or in follow-up studies, diagnostic kits will be provided to laboratories in endemic regions for beta testing. Specific Aim 1 will optimize the selection of oligos used in isothermal amplification of targeted regions and optimize the lysis process to expose the DNA. Because reactants from one step of the process flow into the next chamber, it will be ensured that the buffer compositions are compatible. Specific Aim 2 will optimize the CRISPR/Cas12a reactions for activation of the endonuclease activity upon sensing the target sequences. This Aim will assess the use of multiple targets and determine assay specificity and sensitivity. Specific Aim 3 will develop the configuration of the FAST device and plan future manufacturing scale-up.
Jun 2024 — May 2026
$297,761
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