NGS – Quality Control, Alignment, Visualisation

In many domains of life sciences, Next Generation Sequencing (NGS) methods have become an indispensable part of research and clinical practice. Due to its massive throughput and versatile applications, like genomics, transcriptomics, epigenomics and metagenomics, it supports knowledge generation in a broad range of applications. However, sequencing experiments result in (very) large datasets, with specific file formats, which can make the downstream analysis challenging.

In this course, you will learn about the background of most used sequencing technologies and about the concepts of quality control and read alignment. You will put this knowledge into practice by performing hands-on exercises in which you will learn how to perform such analysis with the command line. To make interpretation of the analysis results easier, you will learn how visualize the output using a genome browser. After performing all analysis steps in a guided manner, you will work on a mini project to learn how to apply the learnt skills more independently.

If you have some experience with the command line, and are ready to get started with NGS analysis, this course is for you.

This course is currently available free of charge as a self-paced, asynchronous learning experience. All course materials can be accessed at any time via the dedicated GitHub page. However, when the course is offered as a synchronous, instructor-led session through the institute, a registration fee will apply. Registered participants of the instructor-led course will receive specific streaming access and detailed instructions directly from the course organizers. Upon successful participation in the synchronous course, participants will also receive a certificate of participation.

This course is available in English only.